Электронный каталог

3
K42
Khan, S. G.
    Research and application of methods for calculating the measurement uncertainty of industrial flowmeters [Текст] / S. G. Khan, L. K. Ibrayeva, N. V. Syabina, Z. M. Yuldashev // Геология және техника ғылымдар сериясы. - 2020. - №6. - P. 145-150
ББК 3

Рубрики: Technique

Кл.слова (ненормированные):
Measurement uncertainty -- Standard GUM method -- Monte Carlo method -- Kragten matrix -- Verification
Аннотация: To create conditions for the recognition of Kazakhstan certificates of conformity and the results of product tests an assessment of measurement uncertainty is required
Держатели документа:
WKU
Доп.точки доступа:
Ibrayeva, L.K.
Syabina, N.V.
Yuldashev, Z.M.

48
V11
Vafin, R. R.
    Technology of bovine leukemia virus genodiagnostics in cattle, in produced raw materials and products. [Текст] / R. R. Vafin, Kh. Kh. Gilmanov, A. G. Galstyan, N. S Pryanichnikova [et al.] // News of national academy of sciences of the republic of Kazakhstan. . - 2021. - №1. - P. 119-125
ББК 48

Рубрики: Ветеринария

Кл.слова (ненормированные):
Bovine leukemia virus -- BLV -- cattle -- milk -- PCR -- RFLP -- sequencing -- gene diagnostics -- genotyping -- env-gene
Аннотация: The most important task of the dairy cattle industry is to obtain high quality raw milk. To achieve it, a set of measures is required, including aimed at increasing the biological safety of produced raw materials. The aim of the study was to create a scientific and methodological basis for the Bovine leukemia virus (BLV) gene diagnostics in a combined format of pathogen indication and identification. This required updating the strategy of BLVPCR-RFLP genotyping, consistent with its phylogenetic classification, taking into account the growing knowledge about the genetic diversity of 11 genotypes of the studied viral pathogen. When staging nested PCR, oligonucleotide primers were used, which initiate at the final stage of the reaction the production of a 444 bp env-gene fragment of the pathogen. Five restriction endonucleases were used in PCR-RFLP BLV genotyping of: PvuII, SspI, AsuHPI, HaeIII, and BstX2I. As a result of verification of the developed Bovine leukemia virus method for gene identification with an updated genotyping strategy, a technical result was obtained, expressed in the ability to identify all 11 BLV genotypes discovered to date by interpreting the generated 58 genotype-associated combinations of PCR-RFLP profiles.
Держатели документа:
WKU
Доп.точки доступа:
Gilmanov, Kh. Kh.
Galstyan, A. G.
Pryanichnikova, N. S
Bigaeva, A. V.
Lazareva, E. G.
Kazakova, V. S.