База данных: Статьи
Страница 11, Результатов: 153
Отмеченные записи: 0
101.
Подробнее
22.3
M99
Myrzatay, A. A.
The effect of the amount of data array on the results of forecasting network equipment failures. [Текст] / A. A. Myrzatay, L. G. Rzayeva, G. A. Uskenbayeva, A. K. Shukirova, G. Abitova // News of national academy of sciences of the republic of Kazakhstan. . - 2021. - №6. - P. 28-36
ББК 22.3
Рубрики: physics
Кл.слова (ненормированные):
machine learning methods -- modeling of machine learning method -- network equipment failure forecasts -- LAN
Аннотация: The article discusses three methods for predicting network equipment failures and the impact of the data array of input controllers. The purpose of the article is to reveal the relevance of the approach proposed by the authors to the use of large-amount of data in the chosen method of machine learning and to make a comparative analysis of the final values with the works of other world researchers. In the first section, the authors analyze the work of scientists from the Beijing University of Post and Telecommunications, noting the strengths and weaknesses of their method. In Section 2, the authors analyze the Holt-Winters method for developing algorithms for analyzing network traffic, which was applied by researchers from the Tomsk State University of Control Systems and Radio Electronics. In section 3, the authors applied the method of random trees in the modeling of machine learning methods of Rapid Miner Studio. The authors have carried out work with a large amount of data, and a comparative analysis of the results of modeling the method. The importance of using large amounts of information to train a model of network equipment failure forecasts is proved. In the final section, the authors highlight the need to improve forecasting models for its further implementation in the working environment. Also, the authors emphasize that the two articles considered by international researchers are a special case, as well as their chosen method for predicting failures in the LAN system.
Держатели документа:
WKU
Доп.точки доступа:
Rzayeva, L.G.
Uskenbayeva, G.A.
Shukirova, A.K.
Abitova, G.
M99
Myrzatay, A. A.
The effect of the amount of data array on the results of forecasting network equipment failures. [Текст] / A. A. Myrzatay, L. G. Rzayeva, G. A. Uskenbayeva, A. K. Shukirova, G. Abitova // News of national academy of sciences of the republic of Kazakhstan. . - 2021. - №6. - P. 28-36
Рубрики: physics
Кл.слова (ненормированные):
machine learning methods -- modeling of machine learning method -- network equipment failure forecasts -- LAN
Аннотация: The article discusses three methods for predicting network equipment failures and the impact of the data array of input controllers. The purpose of the article is to reveal the relevance of the approach proposed by the authors to the use of large-amount of data in the chosen method of machine learning and to make a comparative analysis of the final values with the works of other world researchers. In the first section, the authors analyze the work of scientists from the Beijing University of Post and Telecommunications, noting the strengths and weaknesses of their method. In Section 2, the authors analyze the Holt-Winters method for developing algorithms for analyzing network traffic, which was applied by researchers from the Tomsk State University of Control Systems and Radio Electronics. In section 3, the authors applied the method of random trees in the modeling of machine learning methods of Rapid Miner Studio. The authors have carried out work with a large amount of data, and a comparative analysis of the results of modeling the method. The importance of using large amounts of information to train a model of network equipment failure forecasts is proved. In the final section, the authors highlight the need to improve forecasting models for its further implementation in the working environment. Also, the authors emphasize that the two articles considered by international researchers are a special case, as well as their chosen method for predicting failures in the LAN system.
Держатели документа:
WKU
Доп.точки доступа:
Rzayeva, L.G.
Uskenbayeva, G.A.
Shukirova, A.K.
Abitova, G.
102.
Подробнее
32.973
T22
Tashenova , Zh.
ASSESSMENT OF THE SECURITY STATUS OF THE COMPANY’S DATA CENTER NETWORK INFRASTRUCTURE. [Текст] / Zh. Tashenova , E. Nurlybaeva , Zh. Abdugulova , Sh. Amanzholova // NEWSOF THE NATIONAL ACADEMY OF SCIENCES OF THE REPUBLIC OF KAZAKHSTAN.J2021. - №6. - P. 81-91
ББК 32.973
Рубрики: information Technology
Кл.слова (ненормированные):
network security -- data center -- network -- Cisco -- virtual private server -- proxy servers
Аннотация: The purpose of this article is based on an assessment of the current state of network security in a company’s data center. Analysis of the architecture and design principles of the data center network, development of proposals and recommendations for increasing the level of network security of the company’s data center without serious loss of performance using both existing network elements and additional security elements.
Держатели документа:
WKu
Доп.точки доступа:
Nurlybaeva , E.
Abdugulova , Zh.
Amanzholova , Sh.
T22
Tashenova , Zh.
ASSESSMENT OF THE SECURITY STATUS OF THE COMPANY’S DATA CENTER NETWORK INFRASTRUCTURE. [Текст] / Zh. Tashenova , E. Nurlybaeva , Zh. Abdugulova , Sh. Amanzholova // NEWSOF THE NATIONAL ACADEMY OF SCIENCES OF THE REPUBLIC OF KAZAKHSTAN.J2021. - №6. - P. 81-91
Рубрики: information Technology
Кл.слова (ненормированные):
network security -- data center -- network -- Cisco -- virtual private server -- proxy servers
Аннотация: The purpose of this article is based on an assessment of the current state of network security in a company’s data center. Analysis of the architecture and design principles of the data center network, development of proposals and recommendations for increasing the level of network security of the company’s data center without serious loss of performance using both existing network elements and additional security elements.
Держатели документа:
WKu
Доп.точки доступа:
Nurlybaeva , E.
Abdugulova , Zh.
Amanzholova , Sh.
103.
Подробнее
22.1
I-32
Ibrayev, A. T.
Construction and application of a dynamic motion counting system for researching the properties of electron mirrors and cathode lenses. [Текст] / A. T. Ibrayev // News of national academy of sciences of the republic of Kazakhstan. . - 2021. - №6. - P. 114-120
ББК 22.1
Рубрики: Mathematica
Кл.слова (ненормированные):
motion -- reference frame -- coordinate transformation -- velocity -- interval invariance
Аннотация: Based on the analysis and consideration of the features of Galileo’s transformations, new inertial and dynamic reference frames of movements are obtained. In the new inertial reference frame, the main drawback of Galileo’s transformations, associated with the violation of the invariance of the intervals between events, is eliminated. The developed dynamic frame of reference makes it possible to conduct effective studies of movements in force fields. Elements of this dynamic frame of reference intuitively, without a detailed analysis of the meaning of the transformations used, have been successfully applied in a number of scientific works in the field of physical electronics, devoted to the construction of theories of electron mirrors and cathode lenses. Obviously, the new reference systems indicated in the work will find wide application both in the field of research and design of electron-optical devices and ion-beam technological installations, and in other branches of fundamental and technical sciences.
Держатели документа:
WKU
I-32
Ibrayev, A. T.
Construction and application of a dynamic motion counting system for researching the properties of electron mirrors and cathode lenses. [Текст] / A. T. Ibrayev // News of national academy of sciences of the republic of Kazakhstan. . - 2021. - №6. - P. 114-120
Рубрики: Mathematica
Кл.слова (ненормированные):
motion -- reference frame -- coordinate transformation -- velocity -- interval invariance
Аннотация: Based on the analysis and consideration of the features of Galileo’s transformations, new inertial and dynamic reference frames of movements are obtained. In the new inertial reference frame, the main drawback of Galileo’s transformations, associated with the violation of the invariance of the intervals between events, is eliminated. The developed dynamic frame of reference makes it possible to conduct effective studies of movements in force fields. Elements of this dynamic frame of reference intuitively, without a detailed analysis of the meaning of the transformations used, have been successfully applied in a number of scientific works in the field of physical electronics, devoted to the construction of theories of electron mirrors and cathode lenses. Obviously, the new reference systems indicated in the work will find wide application both in the field of research and design of electron-optical devices and ion-beam technological installations, and in other branches of fundamental and technical sciences.
Держатели документа:
WKU
104.
Подробнее
67.401
A 29
Akhmetov, E. B.
THE IMPORTANCE OF LEGAL PRINCIPLES IN THE SYSTEM OF ADMINISTRATIVE PROCEEDINGS OF THE REPUBLIC OF KAZAKHSTAN [Текст] / E. B. Akhmetov // SCIENTIFIC JOURNAL "THE BULLETIN OF ACADEMY OF LAW ENFORCEMENT AGENCIES". - 2022. - №1. - p. 8-15
ББК 67.401
Рубрики: Әкімшілік құқық
Кл.слова (ненормированные):
administrative proceedings -- administrative procedure -- administrative justice -- universality -- legality -- public administration -- officials -- administrative cases
Аннотация: The article addresses the analysis of the basic principles of administrative proceedings. The main attention is given to the identification of the main functions, the system and the hierarchy of principles. The scientific novelty lies in the study of important subordinate guiding principles, the influence of which is decisive for the principles of administrative proceedings, and hence the entire system of public administration. A brief overview of the basic principles of administrative proceedings and their implementation in the countries of the near and far abroad was made.
Держатели документа:
М. Өтемісов атындағы БҚУ
A 29
Akhmetov, E. B.
THE IMPORTANCE OF LEGAL PRINCIPLES IN THE SYSTEM OF ADMINISTRATIVE PROCEEDINGS OF THE REPUBLIC OF KAZAKHSTAN [Текст] / E. B. Akhmetov // SCIENTIFIC JOURNAL "THE BULLETIN OF ACADEMY OF LAW ENFORCEMENT AGENCIES". - 2022. - №1. - p. 8-15
Рубрики: Әкімшілік құқық
Кл.слова (ненормированные):
administrative proceedings -- administrative procedure -- administrative justice -- universality -- legality -- public administration -- officials -- administrative cases
Аннотация: The article addresses the analysis of the basic principles of administrative proceedings. The main attention is given to the identification of the main functions, the system and the hierarchy of principles. The scientific novelty lies in the study of important subordinate guiding principles, the influence of which is decisive for the principles of administrative proceedings, and hence the entire system of public administration. A brief overview of the basic principles of administrative proceedings and their implementation in the countries of the near and far abroad was made.
Держатели документа:
М. Өтемісов атындағы БҚУ
105.
Подробнее
35
Y46
Yessenamanova, M. S.
The content and distribution of the trace elements in soils during the development of oil fields in the republic of Kalmykia. [Текст] / M. S. Yessenamanova, Ts. D. Davaeva, L. Kh. Sangadzhieva, B. V. Tsombueva [et al.] // News of national academy of sciences of the republic of Kazakhstan. . - 2021. - №1. - P. 14-21
ББК 35
Рубрики: chemical Technology
Кл.слова (ненормированные):
oil-contaminated soils -- heavy metals -- trace elements -- oil products -- drilling sites
Аннотация: The article presents the results of chemical analysis soils of drilling sites of the Sostinsky and Mezhozerny oil fields of the Republic of Kalmykia. The content of sodium ions is more than 2 times higher in both deposits. At the same time, the content of chlorides in some areas of the Mezhozerny field reaches its maximum. As a result, the soils are highly saline in terms of the degree of salinity and are mainly chloride-sulfate-sodium in terms of the type of salinity.
Держатели документа:
WKU
Доп.точки доступа:
Davaeva, Ts.D.
Sangadzhieva, L.Kh.
Tsombueva, B.V.
Sangadzhieva, O.S.
Yessenamanova, Zh.S.
Tlepbergenova, A.E.
Y46
Yessenamanova, M. S.
The content and distribution of the trace elements in soils during the development of oil fields in the republic of Kalmykia. [Текст] / M. S. Yessenamanova, Ts. D. Davaeva, L. Kh. Sangadzhieva, B. V. Tsombueva [et al.] // News of national academy of sciences of the republic of Kazakhstan. . - 2021. - №1. - P. 14-21
Рубрики: chemical Technology
Кл.слова (ненормированные):
oil-contaminated soils -- heavy metals -- trace elements -- oil products -- drilling sites
Аннотация: The article presents the results of chemical analysis soils of drilling sites of the Sostinsky and Mezhozerny oil fields of the Republic of Kalmykia. The content of sodium ions is more than 2 times higher in both deposits. At the same time, the content of chlorides in some areas of the Mezhozerny field reaches its maximum. As a result, the soils are highly saline in terms of the degree of salinity and are mainly chloride-sulfate-sodium in terms of the type of salinity.
Держатели документа:
WKU
Доп.точки доступа:
Davaeva, Ts.D.
Sangadzhieva, L.Kh.
Tsombueva, B.V.
Sangadzhieva, O.S.
Yessenamanova, Zh.S.
Tlepbergenova, A.E.
106.
Подробнее
35
Y46
Yessenamanova, M. S.
Content of heavy metals in the sea water of the northern Caspian Sea. [Текст] / M. S. Yessenamanova // News of national academy of sciences of the republic of Kazakhstan. . - 2021. - №1. - P. 22-29
ББК 35
Рубрики: chemical Technology
Кл.слова (ненормированные):
seawater samples -- heavy metals -- North Caspian deposits -- maximum permissible concentration (MPC) -- water pollution monitoring
Аннотация: The article presents the results of chemical analysis for the content of heavy metals in the waters of the fields in the northern part of the Caspian Sea.
Держатели документа:
WKU
Доп.точки доступа:
Zhakyayeva, D.
Ryszhan, A.
Yessenamanova, Zh.S.
Tlepbergenova, A.E.
Pendashteh, A.
Y46
Yessenamanova, M. S.
Content of heavy metals in the sea water of the northern Caspian Sea. [Текст] / M. S. Yessenamanova // News of national academy of sciences of the republic of Kazakhstan. . - 2021. - №1. - P. 22-29
Рубрики: chemical Technology
Кл.слова (ненормированные):
seawater samples -- heavy metals -- North Caspian deposits -- maximum permissible concentration (MPC) -- water pollution monitoring
Аннотация: The article presents the results of chemical analysis for the content of heavy metals in the waters of the fields in the northern part of the Caspian Sea.
Держатели документа:
WKU
Доп.точки доступа:
Zhakyayeva, D.
Ryszhan, A.
Yessenamanova, Zh.S.
Tlepbergenova, A.E.
Pendashteh, A.
107.
Подробнее
35
A44
Altayeva, A.
Study of gold extraction from stale tailings by agitation leaching. [Текст] / A. Altayeva, B. N. Surimbayev, L. S. Bolotova, Zh. Т. Bagasharova, Sh. K. Akilbekova // News of national academy of sciences of the republic of Kazakhstan. . - 2021. - №1. - P. 89-94
ББК 35
Рубрики: chemical Technology
Кл.слова (ненормированные):
stale tailings -- stale tailings of the processing plant -- leaching -- technogenic mineral formations -- gold
Аннотация: The object of research is technogenic mineral formations-tailings of the Bestube processing plant located within the Bestube gold field. Analytical and technological studies of stale tailings have been carried out. According to the results of analytical studies, the material composition of stale tails was established. The average gold content in stale tailings according to assay analyses is 0.565 g/t, silver 1.56 g/t. The tailings belong to the category of poor sulfide, oxidized raw materials. The main industrially valuable component in the tailings is gold, silver has a subordinate value, and other metals are not of industrial value. Mineralogical analysis established that stale tails are represented by quartz, muscovite, clinochlore, albite, dolomite. Studies were conducted on agitation leaching of stale tailings of the initial size (64.08% of the class –0.074 mm) and after additional grinding to the size of 90% of the class –0.074 mm. Reducing the size contributes to the dissolution of gold from the tailings: at the initial size, 66.28% of gold is dissolved, after regrinding the degree of dissolution of gold from the tailings increases to 87.23%. The results of research on agitation leaching of gold have shown a high efficiency of hydrometallurgical technology for processing stale tailings of a processing plant.
Держатели документа:
WKU
Доп.точки доступа:
Surimbayev, B. N.
Bolotova, L. S.
Bagasharova, Zh. Т.
Akilbekova, Sh. K.
A44
Altayeva, A.
Study of gold extraction from stale tailings by agitation leaching. [Текст] / A. Altayeva, B. N. Surimbayev, L. S. Bolotova, Zh. Т. Bagasharova, Sh. K. Akilbekova // News of national academy of sciences of the republic of Kazakhstan. . - 2021. - №1. - P. 89-94
Рубрики: chemical Technology
Кл.слова (ненормированные):
stale tailings -- stale tailings of the processing plant -- leaching -- technogenic mineral formations -- gold
Аннотация: The object of research is technogenic mineral formations-tailings of the Bestube processing plant located within the Bestube gold field. Analytical and technological studies of stale tailings have been carried out. According to the results of analytical studies, the material composition of stale tails was established. The average gold content in stale tailings according to assay analyses is 0.565 g/t, silver 1.56 g/t. The tailings belong to the category of poor sulfide, oxidized raw materials. The main industrially valuable component in the tailings is gold, silver has a subordinate value, and other metals are not of industrial value. Mineralogical analysis established that stale tails are represented by quartz, muscovite, clinochlore, albite, dolomite. Studies were conducted on agitation leaching of stale tailings of the initial size (64.08% of the class –0.074 mm) and after additional grinding to the size of 90% of the class –0.074 mm. Reducing the size contributes to the dissolution of gold from the tailings: at the initial size, 66.28% of gold is dissolved, after regrinding the degree of dissolution of gold from the tailings increases to 87.23%. The results of research on agitation leaching of gold have shown a high efficiency of hydrometallurgical technology for processing stale tailings of a processing plant.
Держатели документа:
WKU
Доп.точки доступа:
Surimbayev, B. N.
Bolotova, L. S.
Bagasharova, Zh. Т.
Akilbekova, Sh. K.
108.
Подробнее
24
K21
Karmanovskaya, N. V.
Public environmental monitoring of the state of snowpack in norilsk. [Текст] / N. V. Karmanovskaya, O. V. Nosova, A. V. Kaverzin // News of national academy of sciences of the republic of Kazakhstan. . - 2021. - №1. - P. 95-103
ББК 24
Рубрики: chemistry
Кл.слова (ненормированные):
precipitation -- industrial area -- melt water -- chemical characteristics
Аннотация: It is considered that Norilsk ranks second in terms of atmospheric contamination. Industrial enterprises annually emit large amounts of sulphur dioxide, phenols, and heavy metal particles. The city is located within the Far North and is distinguished by the harsh climate of the subarctic type. The snow cover can lie from 244 to 277 days. Snow is a good sorbent; therefore, the snow cover accumulates solid and gaseous pollutants that enter it from the atmosphere with precipitation or are absorbed from it. The purpose of this study was to analyse the snow cover in the Norilsk industrial region and assess the state of atmospheric air in the winter. To achieve this goal, it is necessary to solve the following tasks: conduct a literature review on a given topic; take samples of snow in various industrial districts of Norilsk; study the dependence of the qualitative characteristics of snow on the sampling site; draw conclusions on the quality of snowpack in different areas of the industrial districts of Norilsk. In the course of the study, the following methods were used: organoleptic, visual, methods for determining water transparency, titrimetric analysis, etc. Heavy metal ions and sulphate ions were not detected in the thawed snow, so no industrial gas pollution was noted in the residential area. Most of the particulate contamination was caused by slagging of roads to improve vehicle traction.
Держатели документа:
WKU
Доп.точки доступа:
Nosova, O. V.
Kaverzin, A. V.
K21
Karmanovskaya, N. V.
Public environmental monitoring of the state of snowpack in norilsk. [Текст] / N. V. Karmanovskaya, O. V. Nosova, A. V. Kaverzin // News of national academy of sciences of the republic of Kazakhstan. . - 2021. - №1. - P. 95-103
Рубрики: chemistry
Кл.слова (ненормированные):
precipitation -- industrial area -- melt water -- chemical characteristics
Аннотация: It is considered that Norilsk ranks second in terms of atmospheric contamination. Industrial enterprises annually emit large amounts of sulphur dioxide, phenols, and heavy metal particles. The city is located within the Far North and is distinguished by the harsh climate of the subarctic type. The snow cover can lie from 244 to 277 days. Snow is a good sorbent; therefore, the snow cover accumulates solid and gaseous pollutants that enter it from the atmosphere with precipitation or are absorbed from it. The purpose of this study was to analyse the snow cover in the Norilsk industrial region and assess the state of atmospheric air in the winter. To achieve this goal, it is necessary to solve the following tasks: conduct a literature review on a given topic; take samples of snow in various industrial districts of Norilsk; study the dependence of the qualitative characteristics of snow on the sampling site; draw conclusions on the quality of snowpack in different areas of the industrial districts of Norilsk. In the course of the study, the following methods were used: organoleptic, visual, methods for determining water transparency, titrimetric analysis, etc. Heavy metal ions and sulphate ions were not detected in the thawed snow, so no industrial gas pollution was noted in the residential area. Most of the particulate contamination was caused by slagging of roads to improve vehicle traction.
Держатели документа:
WKU
Доп.точки доступа:
Nosova, O. V.
Kaverzin, A. V.
109.
Подробнее
35
K21
Karmanovskaya , N. V.
Public environmental monitoring of drinking water quality in the city of Norilsk. [Текст] / N. V. Karmanovskaya // News of national academy of sciences of the republic of Kazakhstan. . - 2021. - №1. - P. 104-111
ББК 35
Рубрики: chemical Technology
Кл.слова (ненормированные):
ecological situation -- industrial city -- water supply -- public utilities
Аннотация: According to UN estimates, the shortage of safe drinking water can become one of the most acute and pressing problems. The specificity of this problem for Russia is not a shortage of water resources, but their pollution and ongoing degradation of water bodies. The quality of water is understood as the totality of its properties associated with the nature and concentration of impurities in the water. Water analysis is the only tool for monitoring its condition and properties. During the experiment, a team of researchers took samples of drinking water from the water supply network in various districts of the Norilsk industrial region, studied the dependence of the quality characteristics of water on the location and sampling method. Based on the findings, conclusions on the quality of drinking water were drawn. During the experiment, the authors found that in terms of organoleptic indicators, drinking water in all areas of the NIR meets the requirements of Sanitary Rules and Regulations and GOST. Taking samples at three control points of the Norilsk industrial region, it has been proven that the water is soft and its salt content is mainly due to the presence of hardness salts. Having made the necessary analyses, the authors found that the presence of microorganisms in the water supply network of the NIR is not significant, the permanganate oxidisability indicator does not exceed the requirements of Sanitary Rules and Regulations.
Держатели документа:
WKU
K21
Karmanovskaya , N. V.
Public environmental monitoring of drinking water quality in the city of Norilsk. [Текст] / N. V. Karmanovskaya // News of national academy of sciences of the republic of Kazakhstan. . - 2021. - №1. - P. 104-111
Рубрики: chemical Technology
Кл.слова (ненормированные):
ecological situation -- industrial city -- water supply -- public utilities
Аннотация: According to UN estimates, the shortage of safe drinking water can become one of the most acute and pressing problems. The specificity of this problem for Russia is not a shortage of water resources, but their pollution and ongoing degradation of water bodies. The quality of water is understood as the totality of its properties associated with the nature and concentration of impurities in the water. Water analysis is the only tool for monitoring its condition and properties. During the experiment, a team of researchers took samples of drinking water from the water supply network in various districts of the Norilsk industrial region, studied the dependence of the quality characteristics of water on the location and sampling method. Based on the findings, conclusions on the quality of drinking water were drawn. During the experiment, the authors found that in terms of organoleptic indicators, drinking water in all areas of the NIR meets the requirements of Sanitary Rules and Regulations and GOST. Taking samples at three control points of the Norilsk industrial region, it has been proven that the water is soft and its salt content is mainly due to the presence of hardness salts. Having made the necessary analyses, the authors found that the presence of microorganisms in the water supply network of the NIR is not significant, the permanganate oxidisability indicator does not exceed the requirements of Sanitary Rules and Regulations.
Держатели документа:
WKU
110.
Подробнее
28
V11
Vafin, R. R.
Real-Time pcr technology for cattle genotyping by A and B Kappa -Casein gene alleles. [Текст] / R. R. Vafin // News of national academy of sciences of the republic of Kazakhstan. . - 2021. - №1. - P. 126-132
ББК 28
Рубрики: Biology
Кл.слова (ненормированные):
Bos taurus -- PCR -- RFLP -- genotyping -- allele -- genotype -- CSN3
Аннотация: The main goal of the study was to develop and test an effective technology for cattle genotyping by the CSN3 gene based on real-time PCR with hybridization-fluorescence detection. There was developed a method for real-time PCR for cattle genotyping by A and B alleles of the CSN3 gene in the format of hybridization-fluorescence detection, involving the use of two 5/-fluorescence-labeled forward allele-specific primers, one reverse common primer, and one anti-primer labeled with a fluorescence quencher at the 3/-end of the oligonucleotide. As a result of practical studies aimed at testing the developed method, we obtained the technical result provided by the proposed technology, expressed in the effective identification of the desired genotypes due to correct interpretation of these curves of increasing fluorescence intensity, the results reliability of which was also confirmed by the well-known PCR-RFLP analysis technique for Bos taurus genotyping for similar allelic variants of the kappa-casein gene.
Держатели документа:
WKU
Доп.точки доступа:
Gilmanov, Kh. Kh.
V11
Vafin, R. R.
Real-Time pcr technology for cattle genotyping by A and B Kappa -Casein gene alleles. [Текст] / R. R. Vafin // News of national academy of sciences of the republic of Kazakhstan. . - 2021. - №1. - P. 126-132
Рубрики: Biology
Кл.слова (ненормированные):
Bos taurus -- PCR -- RFLP -- genotyping -- allele -- genotype -- CSN3
Аннотация: The main goal of the study was to develop and test an effective technology for cattle genotyping by the CSN3 gene based on real-time PCR with hybridization-fluorescence detection. There was developed a method for real-time PCR for cattle genotyping by A and B alleles of the CSN3 gene in the format of hybridization-fluorescence detection, involving the use of two 5/-fluorescence-labeled forward allele-specific primers, one reverse common primer, and one anti-primer labeled with a fluorescence quencher at the 3/-end of the oligonucleotide. As a result of practical studies aimed at testing the developed method, we obtained the technical result provided by the proposed technology, expressed in the effective identification of the desired genotypes due to correct interpretation of these curves of increasing fluorescence intensity, the results reliability of which was also confirmed by the well-known PCR-RFLP analysis technique for Bos taurus genotyping for similar allelic variants of the kappa-casein gene.
Держатели документа:
WKU
Доп.точки доступа:
Gilmanov, Kh. Kh.
Страница 11, Результатов: 153