Электронный каталог

48
V11
Vafin, R. R.
    Technology of bovine leukemia virus genodiagnostics in cattle, in produced raw materials and products. [Текст] / R. R. Vafin, Kh. Kh. Gilmanov, A. G. Galstyan, N. S Pryanichnikova [et al.] // News of national academy of sciences of the republic of Kazakhstan. . - 2021. - №1. - P. 119-125
ББК 48

Рубрики: Ветеринария

Кл.слова (ненормированные):
Bovine leukemia virus -- BLV -- cattle -- milk -- PCR -- RFLP -- sequencing -- gene diagnostics -- genotyping -- env-gene
Аннотация: The most important task of the dairy cattle industry is to obtain high quality raw milk. To achieve it, a set of measures is required, including aimed at increasing the biological safety of produced raw materials. The aim of the study was to create a scientific and methodological basis for the Bovine leukemia virus (BLV) gene diagnostics in a combined format of pathogen indication and identification. This required updating the strategy of BLVPCR-RFLP genotyping, consistent with its phylogenetic classification, taking into account the growing knowledge about the genetic diversity of 11 genotypes of the studied viral pathogen. When staging nested PCR, oligonucleotide primers were used, which initiate at the final stage of the reaction the production of a 444 bp env-gene fragment of the pathogen. Five restriction endonucleases were used in PCR-RFLP BLV genotyping of: PvuII, SspI, AsuHPI, HaeIII, and BstX2I. As a result of verification of the developed Bovine leukemia virus method for gene identification with an updated genotyping strategy, a technical result was obtained, expressed in the ability to identify all 11 BLV genotypes discovered to date by interpreting the generated 58 genotype-associated combinations of PCR-RFLP profiles.
Держатели документа:
WKU
Доп.точки доступа:
Gilmanov, Kh. Kh.
Galstyan, A. G.
Pryanichnikova, N. S
Bigaeva, A. V.
Lazareva, E. G.
Kazakova, V. S.

28
V11
Vafin, R. R.
    Real-Time pcr technology for cattle genotyping by A and B Kappa -Casein gene alleles. [Текст] / R. R. Vafin // News of national academy of sciences of the republic of Kazakhstan. . - 2021. - №1. - P. 126-132
ББК 28

Рубрики: Biology

Кл.слова (ненормированные):
Bos taurus -- PCR -- RFLP -- genotyping -- allele -- genotype -- CSN3
Аннотация: The main goal of the study was to develop and test an effective technology for cattle genotyping by the CSN3 gene based on real-time PCR with hybridization-fluorescence detection. There was developed a method for real-time PCR for cattle genotyping by A and B alleles of the CSN3 gene in the format of hybridization-fluorescence detection, involving the use of two 5/-fluorescence-labeled forward allele-specific primers, one reverse common primer, and one anti-primer labeled with a fluorescence quencher at the 3/-end of the oligonucleotide. As a result of practical studies aimed at testing the developed method, we obtained the technical result provided by the proposed technology, expressed in the effective identification of the desired genotypes due to correct interpretation of these curves of increasing fluorescence intensity, the results reliability of which was also confirmed by the well-known PCR-RFLP analysis technique for Bos taurus genotyping for similar allelic variants of the kappa-casein gene.
Держатели документа:
WKU
Доп.точки доступа:
Gilmanov, Kh. Kh.