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База данных: Статьи

Страница 1, Результатов: 11

Отмеченные записи: 0

32.81
B75

Bolatbek, M. A
    Creating the dataset of keywords for detecting an extremist orientation inweb-resources in the Kazakh language [Текст] / M.A Bolatbek // Әл - Фараби ат. ҚҰУ Хабаршы = Вестник КазНУ им Аль - Фараби. - Алматы, 2018. - №1(97). - Р. 134-142. - (Математика, механика, информатика сериясы=Серия математика, механика, информатика. Journal of Mathematics, Mechanics, Computer Science.)
ББК 32.81

Рубрики: Информатика

Кл.слова (ненормированные):
экстремистские тексты -- частота терминов -- классификация текста -- эмоциональные оценка
Аннотация: This paper is part of the research on creating semantic analysis models in web resources for definingan extremist orientation in the text. To solve this task a model was created, which consists of fivestages: identifying websites of an extremist groups, preparing for data extraction, data extraction,data analysis and classification. This work presents the results of data analysis stage of abovementioned model. The purpose of this study is to identify keywords, often used by extremists,which will later be used to classify texts to "extremist" and "neutral" categories using machinelearning methods. There is no such database for the Kazakh language. As the result of this studyan experimental corpus and list of keywords in Kazakh language was created. The keywords wereadded to the database with various morphological variants. The program was built that checksfor the presence of extremist keywords in the given input text and displays the words found.
Держатели документа:
ЗКГУ
Доп.точки доступа:
Mussiraliyeva, Sh.Zh
Tukeyev, U.A

Bolatbek, M.A Creating the dataset of keywords for detecting an extremist orientation inweb-resources in the Kazakh language [Текст] / M.A Bolatbek // Әл - Фараби ат. ҚҰУ Хабаршы = Вестник КазНУ им Аль - Фараби. - Алматы, 2018. - №1(97).- Р.134-142

1.

Bolatbek, M.A Creating the dataset of keywords for detecting an extremist orientation inweb-resources in the Kazakh language [Текст] / M.A Bolatbek // Әл - Фараби ат. ҚҰУ Хабаршы = Вестник КазНУ им Аль - Фараби. - Алматы, 2018. - №1(97).- Р.134-142


32.81
B75

Bolatbek, M. A
    Creating the dataset of keywords for detecting an extremist orientation inweb-resources in the Kazakh language [Текст] / M.A Bolatbek // Әл - Фараби ат. ҚҰУ Хабаршы = Вестник КазНУ им Аль - Фараби. - Алматы, 2018. - №1(97). - Р. 134-142. - (Математика, механика, информатика сериясы=Серия математика, механика, информатика. Journal of Mathematics, Mechanics, Computer Science.)
ББК 32.81

Рубрики: Информатика

Кл.слова (ненормированные):
экстремистские тексты -- частота терминов -- классификация текста -- эмоциональные оценка
Аннотация: This paper is part of the research on creating semantic analysis models in web resources for definingan extremist orientation in the text. To solve this task a model was created, which consists of fivestages: identifying websites of an extremist groups, preparing for data extraction, data extraction,data analysis and classification. This work presents the results of data analysis stage of abovementioned model. The purpose of this study is to identify keywords, often used by extremists,which will later be used to classify texts to "extremist" and "neutral" categories using machinelearning methods. There is no such database for the Kazakh language. As the result of this studyan experimental corpus and list of keywords in Kazakh language was created. The keywords wereadded to the database with various morphological variants. The program was built that checksfor the presence of extremist keywords in the given input text and displays the words found.
Держатели документа:
ЗКГУ
Доп.точки доступа:
Mussiraliyeva, Sh.Zh
Tukeyev, U.A

81.2-2
M44

Maкhazhanova , L. M.
    Classifications of compound words in multi-language systems [Текст] / L. M. Maкhazhanova , L. M. Aliyarova , A.R. Khalenova // Вестник Казахского национального университета имени Аль-Фараби=Bulletin of the Al-Farabi Kazakh national university. - Almaty, 2018. - №4. - Р. 81-85. - (Серия филологическая=Series of рhilology)
ББК 81.2-2

Рубрики: Грамматика

Кл.слова (ненормированные):
сложные слова -- классификация -- взаимоотношение компонентов -- лексико­ грамматический характер -- словообразование -- соединительные элементы -- разносистемные языки -- научные подходы -- языковая система -- правила перевода сложных слов -- многосоставные слова -- немецкая лексика -- свободные словосочетания -- анализ строения сложных имен -- структурные типы -- словообразовательные модели -- грамматические категори­и
Аннотация: В этой статье рассматриваются различные научные подходы и классификации сложных слов в разных языковых системах, а также основные приемы словообразования в немецком языке, способы написания сложных слов, особенности, трудности и правила перевода сложных, многосоставных слов на примерах из немецкой лексики. Целью данной работы является определение отношений сложных имен и изоморфных свободных словосочетаний на основе существующих наиболее приемлемых критериев выделения сложных слов, a также анализ строения сложных имен и установление их основных структурных типов и словообразовательных моделей.В статье дан обзор отечественной и зарубежной литературы по рассматриваемой проблеме, в том числе рассмотрены отзывы отечественных учёных о грамматических категориях. Рассмотренные работы позволили определить основные направления, идеи и понятия в исследовании данного концепта, определить перспективы дальнейшего исследования
Держатели документа:
ЗКГУ
Доп.точки доступа:
Aliyarova , L.M.
Khalenova , A.R.

Maкhazhanova , L.M. Classifications of compound words in multi-language systems [Текст] / L. M. Maкhazhanova , L. M. Aliyarova , A.R. Khalenova // Вестник Казахского национального университета имени Аль-Фараби=Bulletin of the Al-Farabi Kazakh national university. - Almaty, 2018. - №4.- Р.81-85

2.

Maкhazhanova , L.M. Classifications of compound words in multi-language systems [Текст] / L. M. Maкhazhanova , L. M. Aliyarova , A.R. Khalenova // Вестник Казахского национального университета имени Аль-Фараби=Bulletin of the Al-Farabi Kazakh national university. - Almaty, 2018. - №4.- Р.81-85


81.2-2
M44

Maкhazhanova , L. M.
    Classifications of compound words in multi-language systems [Текст] / L. M. Maкhazhanova , L. M. Aliyarova , A.R. Khalenova // Вестник Казахского национального университета имени Аль-Фараби=Bulletin of the Al-Farabi Kazakh national university. - Almaty, 2018. - №4. - Р. 81-85. - (Серия филологическая=Series of рhilology)
ББК 81.2-2

Рубрики: Грамматика

Кл.слова (ненормированные):
сложные слова -- классификация -- взаимоотношение компонентов -- лексико­ грамматический характер -- словообразование -- соединительные элементы -- разносистемные языки -- научные подходы -- языковая система -- правила перевода сложных слов -- многосоставные слова -- немецкая лексика -- свободные словосочетания -- анализ строения сложных имен -- структурные типы -- словообразовательные модели -- грамматические категори­и
Аннотация: В этой статье рассматриваются различные научные подходы и классификации сложных слов в разных языковых системах, а также основные приемы словообразования в немецком языке, способы написания сложных слов, особенности, трудности и правила перевода сложных, многосоставных слов на примерах из немецкой лексики. Целью данной работы является определение отношений сложных имен и изоморфных свободных словосочетаний на основе существующих наиболее приемлемых критериев выделения сложных слов, a также анализ строения сложных имен и установление их основных структурных типов и словообразовательных моделей.В статье дан обзор отечественной и зарубежной литературы по рассматриваемой проблеме, в том числе рассмотрены отзывы отечественных учёных о грамматических категориях. Рассмотренные работы позволили определить основные направления, идеи и понятия в исследовании данного концепта, определить перспективы дальнейшего исследования
Держатели документа:
ЗКГУ
Доп.точки доступа:
Aliyarova , L.M.
Khalenova , A.R.

74
K42

Kharisov, F.
    Use of information and communication technologies in teaching turcik languages in the digital educational environment [Текст] / F. Kharisov, Ch. Kharisova // Bulletin of the Academy of Pedagogical Sciences of Kazakhstan. - 2021. - №4. - P. 36-40
ББК 74

Рубрики: Education

Кл.слова (ненормированные):
education -- information and communication technologies -- educational technologies -- online training
Аннотация: The article provides the classification of ICT tools, describes didactic tasks solved using ICTs, and the advantages of ICTs in teaching Turkic languages.
Держатели документа:
WKU
Доп.точки доступа:
Kharisova, Ch.

Kharisov, F. Use of information and communication technologies in teaching turcik languages in the digital educational environment [Текст] / F. Kharisov, Ch. Kharisova // Bulletin of the Academy of Pedagogical Sciences of Kazakhstan. - 2021. - №4.- P.36-40

3.

Kharisov, F. Use of information and communication technologies in teaching turcik languages in the digital educational environment [Текст] / F. Kharisov, Ch. Kharisova // Bulletin of the Academy of Pedagogical Sciences of Kazakhstan. - 2021. - №4.- P.36-40


74
K42

Kharisov, F.
    Use of information and communication technologies in teaching turcik languages in the digital educational environment [Текст] / F. Kharisov, Ch. Kharisova // Bulletin of the Academy of Pedagogical Sciences of Kazakhstan. - 2021. - №4. - P. 36-40
ББК 74

Рубрики: Education

Кл.слова (ненормированные):
education -- information and communication technologies -- educational technologies -- online training
Аннотация: The article provides the classification of ICT tools, describes didactic tasks solved using ICTs, and the advantages of ICTs in teaching Turkic languages.
Держатели документа:
WKU
Доп.точки доступа:
Kharisova, Ch.

28
D98

Dyulger, G. P.
    Modern methods of diagnosis of mammary tumor and tumor-like lesions in cats [Текст] / G. P. Dyulger, P. G. Dyulger [et al.] // Bulletin of National Academy of Sciences of the Republic of Kazakhstan. - 2021. - №3. - P. 33-39
ББК 28

Рубрики: Biology

Кл.слова (ненормированные):
cats -- mammary glands -- pathology -- feline mammary tumors -- mammare cancer -- diagnosis -- prognostic factors
Аннотация: The paper provides an overview of the classification and diagnosis of feline mammary tumors in cats.
Держатели документа:
WKU
Доп.точки доступа:
Dyulger, P.G.
Alikhanov, O.
Latynina, E.S.
Baimukhanov, D.A.

Dyulger, G.P. Modern methods of diagnosis of mammary tumor and tumor-like lesions in cats [Текст] / G. P. Dyulger, P. G. Dyulger [et al.] // Bulletin of National Academy of Sciences of the Republic of Kazakhstan. - 2021. - №3.- P.33-39

4.

Dyulger, G.P. Modern methods of diagnosis of mammary tumor and tumor-like lesions in cats [Текст] / G. P. Dyulger, P. G. Dyulger [et al.] // Bulletin of National Academy of Sciences of the Republic of Kazakhstan. - 2021. - №3.- P.33-39


28
D98

Dyulger, G. P.
    Modern methods of diagnosis of mammary tumor and tumor-like lesions in cats [Текст] / G. P. Dyulger, P. G. Dyulger [et al.] // Bulletin of National Academy of Sciences of the Republic of Kazakhstan. - 2021. - №3. - P. 33-39
ББК 28

Рубрики: Biology

Кл.слова (ненормированные):
cats -- mammary glands -- pathology -- feline mammary tumors -- mammare cancer -- diagnosis -- prognostic factors
Аннотация: The paper provides an overview of the classification and diagnosis of feline mammary tumors in cats.
Держатели документа:
WKU
Доп.точки доступа:
Dyulger, P.G.
Alikhanov, O.
Latynina, E.S.
Baimukhanov, D.A.

31
C58

Chyrkun, D.
    Intergrated study of the Efficiency of grinding material in an impact -centrifugal mill. [Текст] / D. Chyrkun, A. Levdanskiy, S. Yarmolik, V. Golubev, D. Zhumadullayev // News of national academy of sciences of the republic of Kazakhstan. - 2021. - №3. - P. 129-135
ББК 31

Рубрики: энергетика

Кл.слова (ненормированные):
material -- crushing -- particle -- classification -- device -- fraction -- hydrodynamics -- gravitation -- flowabititty -- flow
Аннотация: The analysis of existing methods of air classification of crushed bulk materials is carried out, priority directions of their development and improvement are identified , the hardware design of these methods and the main approaches to the calculation of air classifiers are stuaied.
Держатели документа:
WKU
Доп.точки доступа:
Levdanskiy, A.
Yarmolik, S.
Golubev, V.
Zhumadullayev, D.

Chyrkun, D. Intergrated study of the Efficiency of grinding material in an impact -centrifugal mill. [Текст] / D. Chyrkun, A. Levdanskiy, S. Yarmolik, V. Golubev, D. Zhumadullayev // News of national academy of sciences of the republic of Kazakhstan. - 2021. - №3.- P.129-135

5.

Chyrkun, D. Intergrated study of the Efficiency of grinding material in an impact -centrifugal mill. [Текст] / D. Chyrkun, A. Levdanskiy, S. Yarmolik, V. Golubev, D. Zhumadullayev // News of national academy of sciences of the republic of Kazakhstan. - 2021. - №3.- P.129-135


31
C58

Chyrkun, D.
    Intergrated study of the Efficiency of grinding material in an impact -centrifugal mill. [Текст] / D. Chyrkun, A. Levdanskiy, S. Yarmolik, V. Golubev, D. Zhumadullayev // News of national academy of sciences of the republic of Kazakhstan. - 2021. - №3. - P. 129-135
ББК 31

Рубрики: энергетика

Кл.слова (ненормированные):
material -- crushing -- particle -- classification -- device -- fraction -- hydrodynamics -- gravitation -- flowabititty -- flow
Аннотация: The analysis of existing methods of air classification of crushed bulk materials is carried out, priority directions of their development and improvement are identified , the hardware design of these methods and the main approaches to the calculation of air classifiers are stuaied.
Держатели документа:
WKU
Доп.точки доступа:
Levdanskiy, A.
Yarmolik, S.
Golubev, V.
Zhumadullayev, D.

22.3
S49

Serebryanskiy, A.
    First results from Tien-Shan survey to search for variable stars: lightcurve analysis and variable star classification [Текст] / A. Serebryanskiy, G. Aimanova, L. Kondrateva, Ch. Omarov // News of the National Academy of sciences of the Republic of Kazakhstan. - 2020. - №3. - Р. 81-91
ББК 22.3

Рубрики: Physics

Кл.слова (ненормированные):
methods:light curve analysis -- photometry -- stars -- variable stars
Аннотация: We present the first results of the photometric survey at Tien-Shan Observatory of the fields around Galactic equator using Zeiss-1000 telescope.
Держатели документа:
WKU
Доп.точки доступа:
Aimanova, G.
Kondrateva, L.
Omarov, Ch.

Serebryanskiy, A. First results from Tien-Shan survey to search for variable stars: lightcurve analysis and variable star classification [Текст] / A. Serebryanskiy, G. Aimanova, L. Kondrateva, Ch. Omarov // News of the National Academy of sciences of the Republic of Kazakhstan. - 2020. - №3.- Р81-91

6.

Serebryanskiy, A. First results from Tien-Shan survey to search for variable stars: lightcurve analysis and variable star classification [Текст] / A. Serebryanskiy, G. Aimanova, L. Kondrateva, Ch. Omarov // News of the National Academy of sciences of the Republic of Kazakhstan. - 2020. - №3.- Р81-91


22.3
S49

Serebryanskiy, A.
    First results from Tien-Shan survey to search for variable stars: lightcurve analysis and variable star classification [Текст] / A. Serebryanskiy, G. Aimanova, L. Kondrateva, Ch. Omarov // News of the National Academy of sciences of the Republic of Kazakhstan. - 2020. - №3. - Р. 81-91
ББК 22.3

Рубрики: Physics

Кл.слова (ненормированные):
methods:light curve analysis -- photometry -- stars -- variable stars
Аннотация: We present the first results of the photometric survey at Tien-Shan Observatory of the fields around Galactic equator using Zeiss-1000 telescope.
Держатели документа:
WKU
Доп.точки доступа:
Aimanova, G.
Kondrateva, L.
Omarov, Ch.

65.47
S26

Savchenko, l.
    Status and classifications of sloganes in advertising communication. [Текст] / l. Savchenko, S. Tovkach, A. Shilina, N. Yablonovskaya // Bulletin of National Academy of Sciences of the Republic of Kazakhstan. - 2021. - №2. - P. 146-153
ББК 65.47

Рубрики: Экономика рекламы

Кл.слова (ненормированные):
slogan -- advertising communication -- advertising text -- information -- advertising language -- emotions -- behavior
Аннотация: The article is devoted to general issues of the functioning of an advertising slogan in the structure of advertising activities ,classification of slogans in advertising communication.
Держатели документа:
WKU
Доп.точки доступа:
Tovkach, S.
Shilina, A.
Yablonovskaya, N.
Subbotina, O.
Burkaltseva, D.
Niyazbekova, Sh.
Vovk, E.

Savchenko, l. Status and classifications of sloganes in advertising communication. [Текст] / l. Savchenko, S. Tovkach, A. Shilina, N. Yablonovskaya // Bulletin of National Academy of Sciences of the Republic of Kazakhstan. - 2021. - №2.- P.146-153

7.

Savchenko, l. Status and classifications of sloganes in advertising communication. [Текст] / l. Savchenko, S. Tovkach, A. Shilina, N. Yablonovskaya // Bulletin of National Academy of Sciences of the Republic of Kazakhstan. - 2021. - №2.- P.146-153


65.47
S26

Savchenko, l.
    Status and classifications of sloganes in advertising communication. [Текст] / l. Savchenko, S. Tovkach, A. Shilina, N. Yablonovskaya // Bulletin of National Academy of Sciences of the Republic of Kazakhstan. - 2021. - №2. - P. 146-153
ББК 65.47

Рубрики: Экономика рекламы

Кл.слова (ненормированные):
slogan -- advertising communication -- advertising text -- information -- advertising language -- emotions -- behavior
Аннотация: The article is devoted to general issues of the functioning of an advertising slogan in the structure of advertising activities ,classification of slogans in advertising communication.
Держатели документа:
WKU
Доп.точки доступа:
Tovkach, S.
Shilina, A.
Yablonovskaya, N.
Subbotina, O.
Burkaltseva, D.
Niyazbekova, Sh.
Vovk, E.

65
M99

Myrkanova, A. M.
    Anisotropy of economic space of the republic of Kazakhstan. [Текст] / A. M. Myrkanova, K. M. Akanova, A. L. Lastovetsky // News of national academy of sciences of the republic of Kazakhstan. . - 2021. - №4. - P. 107-113
ББК 65

Рубрики: Economics

Кл.слова (ненормированные):
anisotropy -- classification of regions -- localization of production -- cluster -- dendrogram
Аннотация: This article is devoted to the study of the rational use of the resource potential of the territories of the Republic of Kazakhstan on the basis of the coefficient of concentration orlocalization of production. The developed mathematical model takes into account many factors affecting the anisotropy of the country's economic space development, and allows us to propose an optimal strategy for managing regions. In the work using a cluster model based on hierarchical algorithms, graphs-dendrograms are constructed and regions are classified according to their level of competitiveness.
Держатели документа:
WKU
Доп.точки доступа:
Akanova, K.M.
Lastovetsky, A.L.

Myrkanova, A.M. Anisotropy of economic space of the republic of Kazakhstan. [Текст] / A. M. Myrkanova, K. M. Akanova, A. L. Lastovetsky // News of national academy of sciences of the republic of Kazakhstan. . - 2021. - №4.- P.107-113

8.

Myrkanova, A.M. Anisotropy of economic space of the republic of Kazakhstan. [Текст] / A. M. Myrkanova, K. M. Akanova, A. L. Lastovetsky // News of national academy of sciences of the republic of Kazakhstan. . - 2021. - №4.- P.107-113


65
M99

Myrkanova, A. M.
    Anisotropy of economic space of the republic of Kazakhstan. [Текст] / A. M. Myrkanova, K. M. Akanova, A. L. Lastovetsky // News of national academy of sciences of the republic of Kazakhstan. . - 2021. - №4. - P. 107-113
ББК 65

Рубрики: Economics

Кл.слова (ненормированные):
anisotropy -- classification of regions -- localization of production -- cluster -- dendrogram
Аннотация: This article is devoted to the study of the rational use of the resource potential of the territories of the Republic of Kazakhstan on the basis of the coefficient of concentration orlocalization of production. The developed mathematical model takes into account many factors affecting the anisotropy of the country's economic space development, and allows us to propose an optimal strategy for managing regions. In the work using a cluster model based on hierarchical algorithms, graphs-dendrograms are constructed and regions are classified according to their level of competitiveness.
Держатели документа:
WKU
Доп.точки доступа:
Akanova, K.M.
Lastovetsky, A.L.

32.973
Y45

Yerassyl, K.
    Sentiment analysis of Kaspi product reviews. [Текст] / K. Yerassyl // News of national academy of sciences of the republic of Kazakhstan. . - 2021. - №5. - P. 68-73
ББК 32.973

Рубрики: information Technology

Кл.слова (ненормированные):
sentiment analysis -- classification -- text analysis -- reviews -- data collection
Аннотация: Customer reviews are the key to the success functionality for the companies. A producer will gain the true result of his products from the customer feedback. What should the seller do to achieve good quality for his product? Just by looking at the stars and reading the usual text, the seller will not be able to improve their product well enough, because different people can understand the same text in different ways. That’s why, the seller can improve the wrong part of their product and make their product worse. Sentimental analysis can be applied here. He can make necessary changes to his product according to the feedback. However, it is common to see when most client may inadequately overestimate or underestimate the mark. To avoid the confusion, it is better to analyze the text. This paper focus on the sentiment feedback analysis and finding the relationship between reviews and ratings on the basis of data collected from ”Kaspi.kz” marketplace. These data streams are cleaned and analyzed and reviews is got through opinion mining. We used several algorithms to find the best one. The experiments have shown 92% accuracy in the sentimental analysis
Держатели документа:
WKU
Доп.точки доступа:
Akhmetov, I.
Jaxylykova, A.

Yerassyl, K. Sentiment analysis of Kaspi product reviews. [Текст] / K. Yerassyl // News of national academy of sciences of the republic of Kazakhstan. . - 2021. - №5.- P.68-73

9.

Yerassyl, K. Sentiment analysis of Kaspi product reviews. [Текст] / K. Yerassyl // News of national academy of sciences of the republic of Kazakhstan. . - 2021. - №5.- P.68-73


32.973
Y45

Yerassyl, K.
    Sentiment analysis of Kaspi product reviews. [Текст] / K. Yerassyl // News of national academy of sciences of the republic of Kazakhstan. . - 2021. - №5. - P. 68-73
ББК 32.973

Рубрики: information Technology

Кл.слова (ненормированные):
sentiment analysis -- classification -- text analysis -- reviews -- data collection
Аннотация: Customer reviews are the key to the success functionality for the companies. A producer will gain the true result of his products from the customer feedback. What should the seller do to achieve good quality for his product? Just by looking at the stars and reading the usual text, the seller will not be able to improve their product well enough, because different people can understand the same text in different ways. That’s why, the seller can improve the wrong part of their product and make their product worse. Sentimental analysis can be applied here. He can make necessary changes to his product according to the feedback. However, it is common to see when most client may inadequately overestimate or underestimate the mark. To avoid the confusion, it is better to analyze the text. This paper focus on the sentiment feedback analysis and finding the relationship between reviews and ratings on the basis of data collected from ”Kaspi.kz” marketplace. These data streams are cleaned and analyzed and reviews is got through opinion mining. We used several algorithms to find the best one. The experiments have shown 92% accuracy in the sentimental analysis
Держатели документа:
WKU
Доп.точки доступа:
Akhmetov, I.
Jaxylykova, A.

48
V11

Vafin, R. R.
    Technology of bovine leukemia virus genodiagnostics in cattle, in produced raw materials and products. [Текст] / R. R. Vafin, Kh. Kh. Gilmanov, A. G. Galstyan, N. S Pryanichnikova [et al.] // News of national academy of sciences of the republic of Kazakhstan. . - 2021. - №1. - P. 119-125
ББК 48

Рубрики: Ветеринария

Кл.слова (ненормированные):
Bovine leukemia virus -- BLV -- cattle -- milk -- PCR -- RFLP -- sequencing -- gene diagnostics -- genotyping -- env-gene
Аннотация: The most important task of the dairy cattle industry is to obtain high quality raw milk. To achieve it, a set of measures is required, including aimed at increasing the biological safety of produced raw materials. The aim of the study was to create a scientific and methodological basis for the Bovine leukemia virus (BLV) gene diagnostics in a combined format of pathogen indication and identification. This required updating the strategy of BLVPCR-RFLP genotyping, consistent with its phylogenetic classification, taking into account the growing knowledge about the genetic diversity of 11 genotypes of the studied viral pathogen. When staging nested PCR, oligonucleotide primers were used, which initiate at the final stage of the reaction the production of a 444 bp env-gene fragment of the pathogen. Five restriction endonucleases were used in PCR-RFLP BLV genotyping of: PvuII, SspI, AsuHPI, HaeIII, and BstX2I. As a result of verification of the developed Bovine leukemia virus method for gene identification with an updated genotyping strategy, a technical result was obtained, expressed in the ability to identify all 11 BLV genotypes discovered to date by interpreting the generated 58 genotype-associated combinations of PCR-RFLP profiles.
Держатели документа:
WKU
Доп.точки доступа:
Gilmanov, Kh. Kh.
Galstyan, A. G.
Pryanichnikova, N. S
Bigaeva, A. V.
Lazareva, E. G.
Kazakova, V. S.

Vafin, R. R. Technology of bovine leukemia virus genodiagnostics in cattle, in produced raw materials and products. [Текст] / R. R. Vafin, Kh. Kh. Gilmanov, A. G. Galstyan, N. S Pryanichnikova [et al.] // News of national academy of sciences of the republic of Kazakhstan. . - 2021. - №1.- P.119-125

10.

Vafin, R. R. Technology of bovine leukemia virus genodiagnostics in cattle, in produced raw materials and products. [Текст] / R. R. Vafin, Kh. Kh. Gilmanov, A. G. Galstyan, N. S Pryanichnikova [et al.] // News of national academy of sciences of the republic of Kazakhstan. . - 2021. - №1.- P.119-125


48
V11

Vafin, R. R.
    Technology of bovine leukemia virus genodiagnostics in cattle, in produced raw materials and products. [Текст] / R. R. Vafin, Kh. Kh. Gilmanov, A. G. Galstyan, N. S Pryanichnikova [et al.] // News of national academy of sciences of the republic of Kazakhstan. . - 2021. - №1. - P. 119-125
ББК 48

Рубрики: Ветеринария

Кл.слова (ненормированные):
Bovine leukemia virus -- BLV -- cattle -- milk -- PCR -- RFLP -- sequencing -- gene diagnostics -- genotyping -- env-gene
Аннотация: The most important task of the dairy cattle industry is to obtain high quality raw milk. To achieve it, a set of measures is required, including aimed at increasing the biological safety of produced raw materials. The aim of the study was to create a scientific and methodological basis for the Bovine leukemia virus (BLV) gene diagnostics in a combined format of pathogen indication and identification. This required updating the strategy of BLVPCR-RFLP genotyping, consistent with its phylogenetic classification, taking into account the growing knowledge about the genetic diversity of 11 genotypes of the studied viral pathogen. When staging nested PCR, oligonucleotide primers were used, which initiate at the final stage of the reaction the production of a 444 bp env-gene fragment of the pathogen. Five restriction endonucleases were used in PCR-RFLP BLV genotyping of: PvuII, SspI, AsuHPI, HaeIII, and BstX2I. As a result of verification of the developed Bovine leukemia virus method for gene identification with an updated genotyping strategy, a technical result was obtained, expressed in the ability to identify all 11 BLV genotypes discovered to date by interpreting the generated 58 genotype-associated combinations of PCR-RFLP profiles.
Держатели документа:
WKU
Доп.точки доступа:
Gilmanov, Kh. Kh.
Galstyan, A. G.
Pryanichnikova, N. S
Bigaeva, A. V.
Lazareva, E. G.
Kazakova, V. S.

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