База данных: Статьи
Страница 10, Результатов: 175
Отмеченные записи: 0
91.
Подробнее
39.5
S28
Sayakov, O.
3D aerodynamic analysis of awing with 2- way fluid-structure interaction. [Текст] / O. Sayakov // News of national academy of sciences of the republic of Kazakhstan. . - 2021. - №4. - P. 129-134
ББК 39.5
Рубрики: Воздушный транспорт
Кл.слова (ненормированные):
NACA 23012 airfoil -- Fluid-Structure Interaction -- ANSYS -- strongly coupling study -- deformation of the wing
Аннотация: the aircraft wings facing different weather conditions start to deform and oscillate with an unpredictable velocity and amplitude. Most of the previous studies were conducted assuming the wing as a rigid body.However, the wing analysis with Fluid-Structure Interaction(FSI)has significant effectson the results. This paper compares the strongly coupled 2-way FSI analysisof the flexible wing with a geometry of NACA 23012and a rigid body 2D winganalysis. Both studies were carried out at a Mach number of 0.075 and Reynolds number of 0.6×106for different angles of attack.Moreover, the analysis of the wing deformation at the start of the flight is also presented. Finally, it was shownthat FSI analysis gives closer results to the experimental measurements, proving the worthiness of the extra efforts of the two-way FSI analysis
Держатели документа:
WKU
Доп.точки доступа:
Zhao, Y.
Mashekova, A.
S28
Sayakov, O.
3D aerodynamic analysis of awing with 2- way fluid-structure interaction. [Текст] / O. Sayakov // News of national academy of sciences of the republic of Kazakhstan. . - 2021. - №4. - P. 129-134
Рубрики: Воздушный транспорт
Кл.слова (ненормированные):
NACA 23012 airfoil -- Fluid-Structure Interaction -- ANSYS -- strongly coupling study -- deformation of the wing
Аннотация: the aircraft wings facing different weather conditions start to deform and oscillate with an unpredictable velocity and amplitude. Most of the previous studies were conducted assuming the wing as a rigid body.However, the wing analysis with Fluid-Structure Interaction(FSI)has significant effectson the results. This paper compares the strongly coupled 2-way FSI analysisof the flexible wing with a geometry of NACA 23012and a rigid body 2D winganalysis. Both studies were carried out at a Mach number of 0.075 and Reynolds number of 0.6×106for different angles of attack.Moreover, the analysis of the wing deformation at the start of the flight is also presented. Finally, it was shownthat FSI analysis gives closer results to the experimental measurements, proving the worthiness of the extra efforts of the two-way FSI analysis
Держатели документа:
WKU
Доп.точки доступа:
Zhao, Y.
Mashekova, A.
92.
Подробнее
22.3
А 13
Абуова, Ф. У.
Структурные , электронные и магнитные свойства n2 CoZ(Ai/GA) при легировании ванадием. [Текст] / Ф. У. Абуова, Т. М. Инербаев, А. У. Абуова, Г. А. Каптагай, Н. Мерәлі // Известия национальной Академии наук РК. - 2021. - №5. - С. 6-16
ББК 22.3
Рубрики: Физика
Кл.слова (ненормированные):
сплавы Гейслера -- ферримагнитное упорядочивание -- бесщелевые спиновые полупроводники -- DFT
Аннотация: в настоящей работе мы исследуем структурные , электронные, магнитные свойства сплавов Гейслера .
Держатели документа:
ЗКУ
Доп.точки доступа:
Инербаев, Т.М.
Абуова, А.У.
Каптагай, Г.А.
Мерәлі, Н.
А 13
Абуова, Ф. У.
Структурные , электронные и магнитные свойства n2 CoZ(Ai/GA) при легировании ванадием. [Текст] / Ф. У. Абуова, Т. М. Инербаев, А. У. Абуова, Г. А. Каптагай, Н. Мерәлі // Известия национальной Академии наук РК. - 2021. - №5. - С. 6-16
Рубрики: Физика
Кл.слова (ненормированные):
сплавы Гейслера -- ферримагнитное упорядочивание -- бесщелевые спиновые полупроводники -- DFT
Аннотация: в настоящей работе мы исследуем структурные , электронные, магнитные свойства сплавов Гейслера .
Держатели документа:
ЗКУ
Доп.точки доступа:
Инербаев, Т.М.
Абуова, А.У.
Каптагай, Г.А.
Мерәлі, Н.
93.
Подробнее
32. 973
S44
Seilova, N. A.
Digital generators of a pseudorandom pulses sequence and their modeling with use of FPGa in the environment cad quartus II. [Текст] / N. A. Seilova, D. Z. Dzhuruntaev, O. Zh. Mamyrbayev, A. B. Batyrgaliev, M. Turdalyuly // News of national academy of sciences of the republic of Kazakhstan. - 2021. - №5. - P. 83-90
ББК 32. 973
Рубрики: information Technology
Кл.слова (ненормированные):
Programmable logic integrated circuit -- digital pseudorandom pulses generators -- rimitive polynomial -- functional modeling -- project compilation
Аннотация: The paper considers the functional modeling of digital generators of a pseudo and ompulses sequence based on FPGAs in the environment of the computer-aided design system (CAD) QUARTUS II by Altera, which supports all stages of designing digital devices based on programmable and reconfigurable logics.
Держатели документа:
WKU
Доп.точки доступа:
Dzhuruntaev, D.Z.
Mamyrbayev, O.Zh.
Batyrgaliev, A.B.
Turdalyuly, M.
S44
Seilova, N. A.
Digital generators of a pseudorandom pulses sequence and their modeling with use of FPGa in the environment cad quartus II. [Текст] / N. A. Seilova, D. Z. Dzhuruntaev, O. Zh. Mamyrbayev, A. B. Batyrgaliev, M. Turdalyuly // News of national academy of sciences of the republic of Kazakhstan. - 2021. - №5. - P. 83-90
Рубрики: information Technology
Кл.слова (ненормированные):
Programmable logic integrated circuit -- digital pseudorandom pulses generators -- rimitive polynomial -- functional modeling -- project compilation
Аннотация: The paper considers the functional modeling of digital generators of a pseudo and ompulses sequence based on FPGAs in the environment of the computer-aided design system (CAD) QUARTUS II by Altera, which supports all stages of designing digital devices based on programmable and reconfigurable logics.
Держатели документа:
WKU
Доп.точки доступа:
Dzhuruntaev, D.Z.
Mamyrbayev, O.Zh.
Batyrgaliev, A.B.
Turdalyuly, M.
94.
Подробнее
22.3
T15
Taimuratova, L. U.
Negative longitudinal magnetoresistance silikon on interline electron transitions. [Текст] / L. U. Taimuratova, O. D. Bigozha, A. Zh. Kazbekova, Z. K. Aimaganbetova // News of national academy of sciences of the republic of Kazakhstan. . - 2021. - №6. - P. 37-41
ББК 22.3
Рубрики: physics
Кл.слова (ненормированные):
piezo resistance -- negative magnetoresistance -- uniaxial pressure -- is energetic -- multivalley model -- valley crossing
Аннотация: The article presents experimental results obtained by studying the magnetoresistance of n-type silicon with an electron concentration of. nе=3,1-1013см-3 in the temperature range of 77.4-300K.There is a negative longitudinal magnetoresistance due to the redistribution of electrons between thevalleys. Experimental results are compared with existing theories and a good agreement is obtained bothquantitatively and qualitatively. At H=350 kE, the energy gap between the lowest Landau subzones is 7 MeV.These experimental results obtained in n-type silicon clearly demonstrate the reliability of the mechanismof negative magnetoresistance caused, indeed, by the pumping of current carriers between the valleys of theconduction band, shifting along the energy scale in a quantizing magnetic field at different speeds. The isenergetic surface of the bottom of the silicon conduction band consists of six ellipsoids located on the mainaxes of the cube, which causes anisotropy of the effective mass and relaxation time. The latter determines thefeatures of galvanomagnetic effects. Especially magnetoresistance is the most sensitive to the anisotropy ofthe is energetic surface. The influence of the latter on magnetoresistance is most clearly revealed in the regionof strong magnetic fields, where magnetoresistance is saturated.
Держатели документа:
WKU
Доп.точки доступа:
Bigozha, O.D.
Kazbekova, A. Zh.
Aimaganbetova, Z.K.
T15
Taimuratova, L. U.
Negative longitudinal magnetoresistance silikon on interline electron transitions. [Текст] / L. U. Taimuratova, O. D. Bigozha, A. Zh. Kazbekova, Z. K. Aimaganbetova // News of national academy of sciences of the republic of Kazakhstan. . - 2021. - №6. - P. 37-41
Рубрики: physics
Кл.слова (ненормированные):
piezo resistance -- negative magnetoresistance -- uniaxial pressure -- is energetic -- multivalley model -- valley crossing
Аннотация: The article presents experimental results obtained by studying the magnetoresistance of n-type silicon with an electron concentration of. nе=3,1-1013см-3 in the temperature range of 77.4-300K.There is a negative longitudinal magnetoresistance due to the redistribution of electrons between thevalleys. Experimental results are compared with existing theories and a good agreement is obtained bothquantitatively and qualitatively. At H=350 kE, the energy gap between the lowest Landau subzones is 7 MeV.These experimental results obtained in n-type silicon clearly demonstrate the reliability of the mechanismof negative magnetoresistance caused, indeed, by the pumping of current carriers between the valleys of theconduction band, shifting along the energy scale in a quantizing magnetic field at different speeds. The isenergetic surface of the bottom of the silicon conduction band consists of six ellipsoids located on the mainaxes of the cube, which causes anisotropy of the effective mass and relaxation time. The latter determines thefeatures of galvanomagnetic effects. Especially magnetoresistance is the most sensitive to the anisotropy ofthe is energetic surface. The influence of the latter on magnetoresistance is most clearly revealed in the regionof strong magnetic fields, where magnetoresistance is saturated.
Держатели документа:
WKU
Доп.точки доступа:
Bigozha, O.D.
Kazbekova, A. Zh.
Aimaganbetova, Z.K.
95.
Подробнее
22.1
S22
Sartabanov, Zh. A.
Mutual reduction of linear multiperiodic systems of equations with differentiation operators. [Текст] / Zh. A. Sartabanov, G. M. Aitenova , G. S. Toremuratova // News of national academy of sciences of the republic of Kazakhstan. . - 2021. - №6. - P. 128-135
ББК 22.1
Рубрики: Mathematica
Кл.слова (ненормированные):
reducibility -- mutual reducibility -- systems -- operator of differentiation -- differentiation along the diagonal -- multiperiodicity -- monodromy
Аннотация: This article is devoted to the study of the problem of mutual reducibility of linear homogeneous systems with multiperiodic coefficients, where a system with a differentiation operator along the direction of the main diagonal is first considered, and then the results of this particular case are generalized to the general case.
Держатели документа:
WKU
Доп.точки доступа:
Aitenova , G.M.
Toremuratova, G.S.
S22
Sartabanov, Zh. A.
Mutual reduction of linear multiperiodic systems of equations with differentiation operators. [Текст] / Zh. A. Sartabanov, G. M. Aitenova , G. S. Toremuratova // News of national academy of sciences of the republic of Kazakhstan. . - 2021. - №6. - P. 128-135
Рубрики: Mathematica
Кл.слова (ненормированные):
reducibility -- mutual reducibility -- systems -- operator of differentiation -- differentiation along the diagonal -- multiperiodicity -- monodromy
Аннотация: This article is devoted to the study of the problem of mutual reducibility of linear homogeneous systems with multiperiodic coefficients, where a system with a differentiation operator along the direction of the main diagonal is first considered, and then the results of this particular case are generalized to the general case.
Держатели документа:
WKU
Доп.точки доступа:
Aitenova , G.M.
Toremuratova, G.S.
96.
Подробнее
24
K70
Koleskin, V. N.
The solving of the problem in the working zone. The numerical calculation of the model of the nizhnekamsk reactor for the styene production (Part -3). [Текст] / V. N. Koleskin // News of the national academy of sciences of the Kazakhstan. . - 2021. - №1. - P. 6-13
ББК 24
Рубрики: chemistry
Кл.слова (ненормированные):
chemical reactor -- still granular layer -- catalyst -- ergun law -- stream function -- granular layer resistance factor -- Green's function pressure field -- velocity field -- layer resistance
Аннотация: The article deals with a mathematical modeling of an incompressible liquidflow in flat and radial contact units with the still granular layer and a creation on numerical realzation methods for the model.
Держатели документа:
WKU
Доп.точки доступа:
Yunusov, A.A.
Yunusova, A.A.
Shtern, P.G.
Lukyanova, A.V.
Zhmadullayev, D.K.
Ainamkulova, T.S.
Bugibaev, E.T.
K70
Koleskin, V. N.
The solving of the problem in the working zone. The numerical calculation of the model of the nizhnekamsk reactor for the styene production (Part -3). [Текст] / V. N. Koleskin // News of the national academy of sciences of the Kazakhstan. . - 2021. - №1. - P. 6-13
Рубрики: chemistry
Кл.слова (ненормированные):
chemical reactor -- still granular layer -- catalyst -- ergun law -- stream function -- granular layer resistance factor -- Green's function pressure field -- velocity field -- layer resistance
Аннотация: The article deals with a mathematical modeling of an incompressible liquidflow in flat and radial contact units with the still granular layer and a creation on numerical realzation methods for the model.
Держатели документа:
WKU
Доп.точки доступа:
Yunusov, A.A.
Yunusova, A.A.
Shtern, P.G.
Lukyanova, A.V.
Zhmadullayev, D.K.
Ainamkulova, T.S.
Bugibaev, E.T.
97.
Подробнее
74
Г 92
Грязнов, С. А.
О роли искусственного интеллекта в современном образовании [Текст] / С. А. Грязнов // Инновации в образовании. - 2022. - №3 Март. - С. 61-68.
ББК 74
Рубрики: Образование
Кл.слова (ненормированные):
Искусственный интеллект в образовании -- AIED -- образовательные инновационные приложения -- адаптируемая система обучения -- актуальные вопросы исследования ИИ в образовании -- масштабируемые технологии
Аннотация: В статье рассмотрены возможности и проблемы, которые искусственный интеллект, посредством инновационных образовательных приложений, открываем перед педагогами; также автором исследуется потенциал данных приложений, вполне способный сделать их новой нормой образования
Держатели документа:
ЗКУ
Г 92
Грязнов, С. А.
О роли искусственного интеллекта в современном образовании [Текст] / С. А. Грязнов // Инновации в образовании. - 2022. - №3 Март. - С. 61-68.
Рубрики: Образование
Кл.слова (ненормированные):
Искусственный интеллект в образовании -- AIED -- образовательные инновационные приложения -- адаптируемая система обучения -- актуальные вопросы исследования ИИ в образовании -- масштабируемые технологии
Аннотация: В статье рассмотрены возможности и проблемы, которые искусственный интеллект, посредством инновационных образовательных приложений, открываем перед педагогами; также автором исследуется потенциал данных приложений, вполне способный сделать их новой нормой образования
Держатели документа:
ЗКУ
98.
Подробнее
28
K42
Khalmurzayeva, B. A.
Biochemical indicators and application of Cyanobacteria Spirulina Microalgae in the cattle feedstuff. [Текст] / B. A. Khalmurzayeva, B. Sh. Ismailkhodjaev, K. P. Kuatbekova, R. S. Alibekov [et al.] // News of national academy of sciences of the republic of Kazakhstan. . - 2021. - №1. - P. 80-88
ББК 28
Рубрики: Biology
Кл.слова (ненормированные):
antioxidants -- biologically active substances -- cultivation -- genus -- microalgae -- phyto-cultures -- Spirulina
Аннотация: The qualitative and quantitative compositions of the valuable biologically active substances, species and strains of microalgae are related the genus of CyanobacteriaSp.platensis and Sp.labgrinthifosmis that were isolated from local nature and grown in semi-production facilities were considered. In order to adapt for mass cultivation, algae were first grown in greenhouse conditions. The algae were cultivated within 12 days. The produc-tivity in the tubular cultivator with stirring of Sp.platensis was 3.8 g / l, protein (73.0%), and Sp.laberinthiformis was 2.6 g / l of protein (63.1%). Similar studies were carried out in the open-air conditions that showed an increase in the amount of illumination causes a significant restructuring of the entire photosynthetic apparatus of algae. The accumulation of Sp.labyrinthiformis 2.7 g / l a.d.c., protein 62.21%, and Sp.platensis 4.08 g / l, protein 75.3%, were within 10 days. The content of protein and carotenes sharply increases in comparison with the greenhouse conditions. It was found that the addition of Sp.platensis suspension, as a protein-vitamin supplement for the bulls’ feedstuff, stimulates physiological processes that improves the biochemical parameters of their blood, the digestibility coefficient of nutrients by 8-9%, positive mineral balance, increases live weight gain (17.4%) and slaughter yield of the bulls (3.6%). So, the suspension of Sp.platensis is recommended for use as a protein-vitamin supplement in livestock farms that will allow having a significant economic effect in livestock farming, in average 20-25% profits per yearly.
Держатели документа:
WKU
Доп.точки доступа:
Ismailkhodjaev, B. Sh.
Kuatbekova, K. P.
Alibekov, R. S.
Makhatova, A. I.
Meimanbaeva, S. S.
K42
Khalmurzayeva, B. A.
Biochemical indicators and application of Cyanobacteria Spirulina Microalgae in the cattle feedstuff. [Текст] / B. A. Khalmurzayeva, B. Sh. Ismailkhodjaev, K. P. Kuatbekova, R. S. Alibekov [et al.] // News of national academy of sciences of the republic of Kazakhstan. . - 2021. - №1. - P. 80-88
Рубрики: Biology
Кл.слова (ненормированные):
antioxidants -- biologically active substances -- cultivation -- genus -- microalgae -- phyto-cultures -- Spirulina
Аннотация: The qualitative and quantitative compositions of the valuable biologically active substances, species and strains of microalgae are related the genus of CyanobacteriaSp.platensis and Sp.labgrinthifosmis that were isolated from local nature and grown in semi-production facilities were considered. In order to adapt for mass cultivation, algae were first grown in greenhouse conditions. The algae were cultivated within 12 days. The produc-tivity in the tubular cultivator with stirring of Sp.platensis was 3.8 g / l, protein (73.0%), and Sp.laberinthiformis was 2.6 g / l of protein (63.1%). Similar studies were carried out in the open-air conditions that showed an increase in the amount of illumination causes a significant restructuring of the entire photosynthetic apparatus of algae. The accumulation of Sp.labyrinthiformis 2.7 g / l a.d.c., protein 62.21%, and Sp.platensis 4.08 g / l, protein 75.3%, were within 10 days. The content of protein and carotenes sharply increases in comparison with the greenhouse conditions. It was found that the addition of Sp.platensis suspension, as a protein-vitamin supplement for the bulls’ feedstuff, stimulates physiological processes that improves the biochemical parameters of their blood, the digestibility coefficient of nutrients by 8-9%, positive mineral balance, increases live weight gain (17.4%) and slaughter yield of the bulls (3.6%). So, the suspension of Sp.platensis is recommended for use as a protein-vitamin supplement in livestock farms that will allow having a significant economic effect in livestock farming, in average 20-25% profits per yearly.
Держатели документа:
WKU
Доп.точки доступа:
Ismailkhodjaev, B. Sh.
Kuatbekova, K. P.
Alibekov, R. S.
Makhatova, A. I.
Meimanbaeva, S. S.
99.
Подробнее
24
K21
Karmanovskaya, N. V.
Public environmental monitoring of the state of snowpack in norilsk. [Текст] / N. V. Karmanovskaya, O. V. Nosova, A. V. Kaverzin // News of national academy of sciences of the republic of Kazakhstan. . - 2021. - №1. - P. 95-103
ББК 24
Рубрики: chemistry
Кл.слова (ненормированные):
precipitation -- industrial area -- melt water -- chemical characteristics
Аннотация: It is considered that Norilsk ranks second in terms of atmospheric contamination. Industrial enterprises annually emit large amounts of sulphur dioxide, phenols, and heavy metal particles. The city is located within the Far North and is distinguished by the harsh climate of the subarctic type. The snow cover can lie from 244 to 277 days. Snow is a good sorbent; therefore, the snow cover accumulates solid and gaseous pollutants that enter it from the atmosphere with precipitation or are absorbed from it. The purpose of this study was to analyse the snow cover in the Norilsk industrial region and assess the state of atmospheric air in the winter. To achieve this goal, it is necessary to solve the following tasks: conduct a literature review on a given topic; take samples of snow in various industrial districts of Norilsk; study the dependence of the qualitative characteristics of snow on the sampling site; draw conclusions on the quality of snowpack in different areas of the industrial districts of Norilsk. In the course of the study, the following methods were used: organoleptic, visual, methods for determining water transparency, titrimetric analysis, etc. Heavy metal ions and sulphate ions were not detected in the thawed snow, so no industrial gas pollution was noted in the residential area. Most of the particulate contamination was caused by slagging of roads to improve vehicle traction.
Держатели документа:
WKU
Доп.точки доступа:
Nosova, O. V.
Kaverzin, A. V.
K21
Karmanovskaya, N. V.
Public environmental monitoring of the state of snowpack in norilsk. [Текст] / N. V. Karmanovskaya, O. V. Nosova, A. V. Kaverzin // News of national academy of sciences of the republic of Kazakhstan. . - 2021. - №1. - P. 95-103
Рубрики: chemistry
Кл.слова (ненормированные):
precipitation -- industrial area -- melt water -- chemical characteristics
Аннотация: It is considered that Norilsk ranks second in terms of atmospheric contamination. Industrial enterprises annually emit large amounts of sulphur dioxide, phenols, and heavy metal particles. The city is located within the Far North and is distinguished by the harsh climate of the subarctic type. The snow cover can lie from 244 to 277 days. Snow is a good sorbent; therefore, the snow cover accumulates solid and gaseous pollutants that enter it from the atmosphere with precipitation or are absorbed from it. The purpose of this study was to analyse the snow cover in the Norilsk industrial region and assess the state of atmospheric air in the winter. To achieve this goal, it is necessary to solve the following tasks: conduct a literature review on a given topic; take samples of snow in various industrial districts of Norilsk; study the dependence of the qualitative characteristics of snow on the sampling site; draw conclusions on the quality of snowpack in different areas of the industrial districts of Norilsk. In the course of the study, the following methods were used: organoleptic, visual, methods for determining water transparency, titrimetric analysis, etc. Heavy metal ions and sulphate ions were not detected in the thawed snow, so no industrial gas pollution was noted in the residential area. Most of the particulate contamination was caused by slagging of roads to improve vehicle traction.
Держатели документа:
WKU
Доп.точки доступа:
Nosova, O. V.
Kaverzin, A. V.
100.
Подробнее
48
V11
Vafin, R. R.
Technology of bovine leukemia virus genodiagnostics in cattle, in produced raw materials and products. [Текст] / R. R. Vafin, Kh. Kh. Gilmanov, A. G. Galstyan, N. S Pryanichnikova [et al.] // News of national academy of sciences of the republic of Kazakhstan. . - 2021. - №1. - P. 119-125
ББК 48
Рубрики: Ветеринария
Кл.слова (ненормированные):
Bovine leukemia virus -- BLV -- cattle -- milk -- PCR -- RFLP -- sequencing -- gene diagnostics -- genotyping -- env-gene
Аннотация: The most important task of the dairy cattle industry is to obtain high quality raw milk. To achieve it, a set of measures is required, including aimed at increasing the biological safety of produced raw materials. The aim of the study was to create a scientific and methodological basis for the Bovine leukemia virus (BLV) gene diagnostics in a combined format of pathogen indication and identification. This required updating the strategy of BLVPCR-RFLP genotyping, consistent with its phylogenetic classification, taking into account the growing knowledge about the genetic diversity of 11 genotypes of the studied viral pathogen. When staging nested PCR, oligonucleotide primers were used, which initiate at the final stage of the reaction the production of a 444 bp env-gene fragment of the pathogen. Five restriction endonucleases were used in PCR-RFLP BLV genotyping of: PvuII, SspI, AsuHPI, HaeIII, and BstX2I. As a result of verification of the developed Bovine leukemia virus method for gene identification with an updated genotyping strategy, a technical result was obtained, expressed in the ability to identify all 11 BLV genotypes discovered to date by interpreting the generated 58 genotype-associated combinations of PCR-RFLP profiles.
Держатели документа:
WKU
Доп.точки доступа:
Gilmanov, Kh. Kh.
Galstyan, A. G.
Pryanichnikova, N. S
Bigaeva, A. V.
Lazareva, E. G.
Kazakova, V. S.
V11
Vafin, R. R.
Technology of bovine leukemia virus genodiagnostics in cattle, in produced raw materials and products. [Текст] / R. R. Vafin, Kh. Kh. Gilmanov, A. G. Galstyan, N. S Pryanichnikova [et al.] // News of national academy of sciences of the republic of Kazakhstan. . - 2021. - №1. - P. 119-125
Рубрики: Ветеринария
Кл.слова (ненормированные):
Bovine leukemia virus -- BLV -- cattle -- milk -- PCR -- RFLP -- sequencing -- gene diagnostics -- genotyping -- env-gene
Аннотация: The most important task of the dairy cattle industry is to obtain high quality raw milk. To achieve it, a set of measures is required, including aimed at increasing the biological safety of produced raw materials. The aim of the study was to create a scientific and methodological basis for the Bovine leukemia virus (BLV) gene diagnostics in a combined format of pathogen indication and identification. This required updating the strategy of BLVPCR-RFLP genotyping, consistent with its phylogenetic classification, taking into account the growing knowledge about the genetic diversity of 11 genotypes of the studied viral pathogen. When staging nested PCR, oligonucleotide primers were used, which initiate at the final stage of the reaction the production of a 444 bp env-gene fragment of the pathogen. Five restriction endonucleases were used in PCR-RFLP BLV genotyping of: PvuII, SspI, AsuHPI, HaeIII, and BstX2I. As a result of verification of the developed Bovine leukemia virus method for gene identification with an updated genotyping strategy, a technical result was obtained, expressed in the ability to identify all 11 BLV genotypes discovered to date by interpreting the generated 58 genotype-associated combinations of PCR-RFLP profiles.
Держатели документа:
WKU
Доп.точки доступа:
Gilmanov, Kh. Kh.
Galstyan, A. G.
Pryanichnikova, N. S
Bigaeva, A. V.
Lazareva, E. G.
Kazakova, V. S.
Страница 10, Результатов: 175